In 2017, the National HIV program implemented the “test and treat” policy which provided antiretroviral treatment (ART) to all people living with HIV (PLHIV) regardless of the CD4 counts. Then followed the expansion of the community-based testing with lay provider testing, selftesting and PrEP services for high risk population. At the same time, rapid expansion of viral load testing services, increased HIV testing of the pregnant women, expansion of ART centers further improved the coverage and quality of the HIV care. Since the inception of Nepal’s anti-retroviral treatment programme in 2O04, early diagnosis with innovative and quality assured methods, effective drugs and quality health services has been the key to successful HIV program in Nepal. This new National HIV testing and treatment guidelines, 2020 refnes the testing strategy for the infants, adults and pregnant, introduces DTG as the frst line drugs for all and advocates on rapid initiation of ART.